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NMRView issues with saving RNA assignments in STAR file



Since I recently joined the list, I don't know if this has been addressed prior, so I'll get it out in the open. The background specifics: I'm using NMRViewJ 6.6.2 with Java version 5. I recently began to assign sugar proton resonances for an RNA that I'm working with. I entered all of the shifts that I had into the atom assignment table and saved everything in a STAR file. When I tried to read the STAR file in NMRViewJ, I got an error. It turns out that the apostrophe (prime) that is used to denote the sugar atoms is also used for commenting out stuff in the STAR file. Therefore, when NMRViewJ was attempting to read the STAR file, it was disregarding the chemical shifts and constraints that appeared on the line of text after the apostrophe (prime). I changed all of the apostrophes to a lower case s in my STAR file as well as all of the nucleotide reslib files, and it seems to work just fine.

John Means
Ohio University